Cubosomes are the square and round shaped particles with internal visible cubic lattices. The discovery of cubosomes is a unique story and spans the field of food science, differential. Cubosomes are nanoparticles but instead of the solid particles usually encountered, cubosomes are selfassembled liquid crystalline particles with a solidlike. The particle size of the cubosomes was determined through dynamic light scattering dls, brookhaven instruments corporation, austin, tx, usa. Interaction of cubosomes with plasma components resulting in the destabilization of cubosomes in plasma. The discovery of cubosomes is an extraordinary story and spans the fields of food science, differential.
Full text characterization of cubosomes as a targeted and. Comparision of the physical characteristics of cubosomes prepared using different manufacturing methods marta ruano aldea, university of strathclyde, uk special session 16. Surfactants lipids polymers amphiphiles in polar solvents the hydrophobic effect drives them to spontaneously self assemble into a rich array of thermodynamically stable lyotropic liquid crystalline phases with characteristic lengths on the nanometer scale. Dls, sometimes referred to as photon correlation spectroscopy, which is a noninvasive, wellestablished technique for measuring the size of molecules and particles typically in the submicron region. Structure of cubosome separating two internal aqueous channels along with large interfacial area advertisement. Decreasing the chitosan concentration increased the gelation time to 1520 min for the 1% formulation compared to download pdf download. Soft selfassembled drug nanocarriers based on cubosomes.
Burn is a painful event that can affect several organ systems as well as the skin and may cause morbidity and mortality. Sarode master of science biomedical and pharmaceutical sciences university of rhode island 2. Characterization of cubosomes as a targeted and sustained. Cubosomes are formed at controlled temperatures into lipid bilayer twisted into three dimension with minimal surface forming a tightly packed structure with bicontinuous domains of water and lipid. Download pdf introduction cubic crystalline single networks of large openspace lattices are ubiquitous in nature, from biophotonic crystals such as the wing scales of butterflies to the cuticles. Biomathematics download ebook pdf, epub, tuebl, mobi. Dynamic visualization of dendritic cellantigen interactions. A novel drug delivery system 26 volume 2 number 1 feb 2012 3. Introduction delivery of drugs to the ocular sight is a promising field for the formulation researchers. Abstract cubosomes are the square and round shaped particles with internal visible cubic lattices. The vesicle is composed of a bilayer of nonionic surface active agents and hence the name niosomes. The cubosomes at room temperature is by diluting monooleinethanol solution with aqueouspoloxamer 407 solution.
Modified thermoresponsive poloxamer 407 and chitosan solgels. The bar equals 200 nm in panel a and 60 nm in panel b. Cubosomes are a versatile new drug delivery platform with potential for high loading of actives with varying physiochemical properties and the potential of sustained release. Niosomes are unilamellar or multilamellar vesicles.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. A diverse range of materials have been used to form niosomes such as sucrose ester surfactants and polyoxyethylene alkyl ether surfactants, alkyl ester, alkyl amides, fatty acids and. Full text characterization of cubosomes as a targeted. It is necessary to add stabilisers such as pluronics in these formulations to circumvent aggregation. Although great progress has been made in this field, burn is still a serious problem in the world and a catastrophic event with patients suffering long hospitalization and rehabilitation. It is likely that this effect is due to the fact that pluronic f127 has a lower affinity for binding to the lipid cubic phase structures in phy cubosomes than gmo cubosomes. The in vivo observation suggested that a long circulation time of an incorporated substance in cubosomes was due to the sustained behavior of cubosome remnant particles. Characterization of cubosomes as a targeted and sustained transdermal delivery system for capsaicin xinsheng peng,1 yanfang zhou,1 ke han,2,3 lingzhen qin,3 linghui dian,1 ge li,4 xin pan,3 chuanbin wu3 1guangdong medical university, dongguan, 2the second affiliated hospital of guangzhou medical university, 3school of pharmaceutical sciences, sun yatsen university, 4guangzhou neworld. Formulation and characterization of cubosomes using lipids and block copolymers ashish l. To find out more, please visit the preparation section below. The cubosomes consisted of commercial glyceryl monooleate, dimodan.
Apr 15, 2004 the destabilization of cubosomes in plasma resulting in smaller particle remnants was as a result of the interaction of the particles with plasma components. The current research work was performed with an objective to deliver dapsone across the skin using cubosomes. Cubosomes are having wide range of applications in various fields and they can be characterized by various. The word bicontinuous refers to the division of the two continuous but nonintersecting aqueous regions by a lipid bilayer that is contorted into a spacefilling structure. Users may download and print one copy of any publication from the public portal. Cubosomal dispersions were prepared by disrupting a cubic gel phase of monoolein and water in the presence of poloxamer 407 as a stabilizer. Fabrication and evaluation of ketorolac loaded cubosome. A very rich variety of aggregates has been found, spanning from vesicles to. The structures of cubosomes of the d, g and p types of minimal surfaces are described by mathematical functions on the exponential scale, and the shape and internal structure of the calculated dtype cubosome is compared to electron microscopy studies. Cryotransmission electron microscopy cryotem, and its technological variations thereof, have become a powerful tool for detailed morphological characterization and 3d tomography of soft lipid and polymeric nanoparticles as well as biological materials such as viruses and dna without chemical fixation. Pdf on oct 23, 2019, sherif gaballa and others published cubosomes. There are three different proposed phases that these cubic structures can be in.
Dec 14, 2018 download pdf introduction cubic crystalline single networks of large openspace lattices are ubiquitous in nature, from biophotonic crystals such as the wing scales of butterflies to the cuticles. Niosomes are a novel drug delivery system, in which the medication is encapsulated in a vesicle. Cubosomes are discrete, submicron, nanostructured particles of the bicontinuous cubic liquid crystalline phase. Another procedure is also developed to produce the cubosomes from powdered precursors by spray drying method. Under certain conditions appropriately selected particles can form closed surfaces in liquids with surprisingly complex shapes, cutting through space by a regular network of channels. These values indicated that the cubosomes possessed a neutral zeta potential due to the usage of poloxamer 407 a non ionic stabilizer and gmo amphoteric lipid. Surfactants lipids polymers amphiphiles in polar solvents the hydrophobic effect drives them to spontaneously self assemble into a rich array of thermodynamically stable lyotropic liquid crystalline phases with. Cubosomes are nanoparticles but instead of the solid particles, cubosomes are selfassembled liquid crystalline particles of certain surfactant with proper ratio of water with a microstructure that provides unique properties of practical interest. Ketorolac tromethamine, glyceryl monooleate, poloxamer 407, ocular drug delivery. T1 cubosomes and hexosomes as versatile platforms for drug delivery. Cubosomes were developed and optimized by utilizing glyceryl monoin vitro characteristics i. Fabrication and evaluation of ketorolac loaded cubosome for. Agerelated strategies in pediatric traumatology martin m kaiser, bg hospital bergmannstrost, germany poster presentations 17. In vitro 5fu release from cubosomes exhibited a phase of rapid release of about half of the entrapped drug during the first hour, followed by a relatively slower drug release as compared to 5fu solution.
This laboratory manual expertly aids students who wish to gain a deeper understanding of solving biological issues with computer programs. Interaction of cubosomes with plasma components resulting in. In order to develop an oral formation of amphotericin b amb using phytantriol pyt based cubosomes with desirable properties, homogenization conditions were firstly investigated to determine their effects on the morphological and dimensional characteristics of cubosomes. Cubosomes and hexosomes as versatile platforms for drug. Anan yaghmur university of copenhagen location tau summer school on nanomedicine and innovation, the marian gertner institute for medical nanosystems, tel aviv university, june 1519. Nonlamellar liquid crystalline phases are attractive platforms for drug solubilization and targeted delivery.
Hydrating a surfactant or polar lipid that forms cubic phase and then dispersing the solidlike phase into smaller particles usually form cubosomes. Abstract cubosomes are lipid bilayer particles that can be formed by dispersing minimal surfaceshaped infinite lipid bilayers in water. Novel vehicle based on cubosomes for ophthalmic delivery of. Aug 03, 2015 cubosomes posses much lower viscosity than the bulk cubic phase,16 and they show better storing stability at room temperature and greater endurance of heat treatment as compared to liposomes. Cubosomes consist of honeycombed cavernous structures separating two internal aqueous.
Microcontainers as an oral delivery system for spray. Soft selfassembled drug nanocarriers based on cubosomes and. Examples of oral drug delivery utilizing cubosomes formulation advertisement. Modified thermoresponsive poloxamer 407 and chitosan sol. Dapsone loaded cubosomes dc were prepared by ultrasonication of aqueous dispersion containing cubic gel matrix of glyceryl monooleate gmo and poloxamer 407. The cubosomes are automatically formed by emulsification. The average zeta potential of dc was in the range of. Hydrating a surfactant or polar lipid that forms cubic phase and then dispersing the solidlike phase. Presentation mode open print download current view.
In cubosomes its their interior that counts sciencedaily. The particle size of each cubosome formulation was about 150 nm. An amphiphilic rutheniumbased molecule dopuru with potential antineoplastic activity has been synthesized, and its aggregation behavior in the presence of phospholipids has been investigated. Marine drugs free fulltext physical stability studies of semisolid formulations from natural compounds loaded with chitosan microspheres html. Bicontinuous cubic phase nanoparticle lipid chemistry. Cubosomes posses much lower viscosity than the bulk cubic phase,16 and they show better storing stability at room temperature and greater endurance of heat treatment as compared to liposomes. Under certain conditions appropriately selected particles can form closed surfaces in liquids with surprisingly complex shapes, cutting through space by a. Find, read and cite all the research you need on researchgate. The attractiveness of this formulation principle is linked to the nanostructural versatility, compatiblity, digestiblity and bioadhesive properties of their lipid constituents, and the capability of solubilizing and sustaining the release of amphiphilic, hydrophobic and hydrophilic drugs. At present, the most common method of preparation of cubosomes involves the mechanical dispersion of the preformed viscous cubic phase. N2 nonlamellar liquid crystalline phases are attractive platforms for drug solubilization and targeted delivery. Abstractdelivery of vaccines into the skin provides many advantages over traditional parenteral vaccination and is a. Chitosan solgels with reduced concentrations of chitosan from 2.
The term bicontinuous refers to two distinct hydrophilic regions separated by the bilayer. This method of production is feasible on a small scale and results in cubosomes which have shown stability against aggregation, in some cases for up to a year. Open symbols represent formulations without cubosomes and crossed or filled symbols represent formulations loaded with cubosomes containing ova, quil a and mpl. Formulation and evaluation of aceclofenac loaded cubosomal. Cubosomes of dapsone enhanced permeation across the skin.
Since the structure of the niosome offers place to accommodate hydrophilic, lipophilic as well as amphiphilic drug moieties, they can be used for a variety of drugs. The remainder of the chapter will then focus on cubosomes, dispersions of the v 2 phase and their. Bicontinuous cubic crystalline materials have been an active research topic because their structure lends itself well to controlledrelease applications. In vitro and in vivo evaluation of cubosomes containing 5. Preparation of phytantriol cubosomes by solvent precursor. Cubosomes are discrete, submicron, nanostructured particles of bicontinuous cubic liquid crystalline phase figure 2. Cubosomes and hexosomes as versatile platforms for drug delivery. Laboratory manual of biomathematics is a companion to the textbook an invitation to biomathematics. Optimization of the preparation process for an oral. Cubosomes with size ranges from 10 to 500 nm and pore size about 510 nm have great potential for encapsulation of diverse types of actives such as hydrophilic, hydrophobic and amphiphilic molecules, and hold excellent promise as drug delivery systems. The aim of present work was to formulate and evaluate sustained release formulation of aceclofenac a non steroidal antiinflammatory drug nsaid, as cubosomal topical gel to reduce gastro intestinal effects and to improve the bioavailability of the drug. The discovery of cubosomes is an extraordinary story and spans the fields of food science, differential geometry.
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